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| 2D Gel Assay for Correct Folding |
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| of Pancreatic Enzymes in RM |
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| RER
sequestration & proteolytic processing required for formation of |
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| disulfide
bonds & correct conformational maturation |
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| Correctly folded
pancreatic enzymes require correct disulfide bond formation to |
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| appear as
distinct spots in 2D gel (Panels b, c & d). |
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| Despite correct
redox potential, presecretory proteins do not form SS bonds and |
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| migrate in distinct spots
(Panel a) |
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